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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Neurosci Res. 2012 Mar 13;90(8):1533–1546. doi: 10.1002/jnr.23041

Figure 5. Localized inhibition of proteasomes results in the accumulation of ubiquitinated proteins in compartmentalized neuronal cultures.

Figure 5

(A–C) Representative western blots for PSMA2 (proteasomes), multi-ubiquitin and total ubiquitin are shown. (D) Inhibition of proteasome function in distal axons (E-DA) or cell bodies (E-CB) resulted in increases in PSMA2 expression. (E–F) Ubiquitinated proteins accumulated in the axons/terminals following proteasome inhibition in the terminals. A modest accumulation of ubiquitinated proteins also occurred in the cell bodies after proteasome inhibition in the cell bodies. Values were normalized to actin and an in-blot vehicle treated control and displayed as mean ± SEM.