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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: J Neurosci Res. 2012 Mar 13;90(8):1533–1546. doi: 10.1002/jnr.23041

Figure 6. Selective inhibition of lysosomes does not cause the accumulation of proteins in compartmentalized neuronal cultures.

Figure 6

ConcanamycinA was used to selectively inhibit lysosomes in either the cell body or distal axon compartments of SCG cultures. (A–B) LAMP2 protein expression was greater in the cell bodies after inhibition of lysosomes in the cell body compartment. LAMP2 expression was also significantly greater in the distal axons (DA) after inhibition of lysosomes in the terminal compartment. No significant accumulation of transferrin receptor (C–D) or total ubiquitin (E–F) was observed following selective lysosome inhibition on either the cell bodies or the distal axons. Bars represent mean ± SEM. P≤0.05 was considered significant by one way ANOVA on ranks. Values were normalized to actin and an in-blot vehicle treated control.