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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: J Mol Cell Cardiol. 2012 Sep 18;53(6):790–800. doi: 10.1016/j.yjmcc.2012.09.005

Figure 2. Early expression of CD34 and CD31 in mouse DFAT cells.

Figure 2

(A) (Left panels) Formation of cell colony formed from one mDFAT cell over the course of 8 days, as shown by phase contrast microscopy. (Right) Expression of CD34 (red fluorescence) is detected on Day 4, co-expression of CD34 and CD31 (red and green fluorescence, respectively) is detected on Day 6, and expression of CD31 (green fluorescence) is detected on Day 8. DAPI (blue) is used to visualize nuclei. Non-specific IgG control showed no staining in the experiments in panel A or B.

(B) (Upper panels) Expression of ASMA (green fluorescence) is detected in mDFAT cells on Day 5 in culture and later. (Lower panels) No co-expression of ASMA and CD31 (red fluorescence) was detected in mDFAT cells.