. Author manuscript; available in PMC: 2013 Feb 1.

Published in final edited form as: Curr Opin Immunol. 2011 Dec 15;24(1):119–128. doi: 10.1016/j.coi.2011.11.005

Table 1.

Early islet cell entry evaluation performed with adoptive transferred specific and non-specific CD4 T cells

CD4 T cell evaluated		Permutation	Recipient strain	Islet entry
	*Manipulation*
3A9^*
	Non-activated	None	IP-HEL	No

	Activated	None	IP-HEL	Yes

	Activated	None	Non-IP-HEL	No

	Activated	Anti-class II	IP-HEL	Reduced

	Activated	Anti-ICAM-1	IP-HEL	Reduced

	Activated	Anti-class II + Anti-ICAM-1	IP-HEL	Reduced

	Activated	Anti-VCAM-1	IP-HEL	Yes

	Activated	Anti-PECAM-1	IP-HEL	Yes

	Activated + PTX	None	IP-HEL	Yes

	Activated	Anti-IFN-γ	IP-HEL	Yes

B10.BR^‡
	Non-activated	None	IP-HEL	No

	Non-activated	None	Non-IP-HEL	No

	Activated	None	IP-HEL	No

	Activated	None	Non-IP-HEL	No

	Activated	STZ	IP-HEL	No

	Non-activated	Co-transfer with 3A9	IP-HEL	Reduced

	Activated	Co-transfer specific	IP-HEL	Yes

	Activated + PTX	Co-transfer specific	IP-HEL	No

	Activated	Co-transfer specific + anti-VCAM-1	IP-HEL	No

	Activated	Co-transfer specific + anti-IFN-γ	IP-HEL	Reduced

BDC2.5^*
	Non-activated	None	NOD.Rag-1^-/-	Reduced

	Activated	None	NOD.Rag-1^-/-	Yes

	Activated	None	B6.g7	Yes

	Activated	None	BALB/c	No

	Activated	None	NOD.Class II^-/-	No

	Activated	Anti-Class II	NOD.Rag-1^-/-	Reduced

	Activated	None	NOD.ICAM-1^-/-	Reduced

	Activated	Anti-Class II	NOD.ICAM-1^-/-	Reduced

	Activated	Anti-CD44	NOD.Rag-1^-/-	Yes

	Activated + PTX	None	NOD.Rag-1^-/-	Yes

	Activated	None	NOD. IFN-γR^-/-	Reduced

B6.g7^‡
	Non-activated	None	NOD.Rag-1^-/-	No

	Non-activated	None	B6.g7	No

	Activated	None	NOD.Rag-1^-/-	No

	Activated	None	B6.g7	No

	Activated	Co-transfer specific	NOD.Rag-1^-/-	Yes

	Activated + PTX	Co-transfer specific	NOD.Rag-1^-/-	No

	Activated	Co-transfer specific + anti-VCAM-1	NOD.Rag-1^-/-	No

	Activated	Co-transfer specific	NOD. IFN-γR^-/-	No

This Table summarized findings of ours reported in references 23 and 24.

3A9 is a CD4 T cell that recognizes the 48-62 peptide of HEL bound to I-A^k molecules. Cells were isolated from a T cell receptor transgenic mice and transferred directly (non-activated), or following a short period of activation with the peptide (activated). Recipients were mice that express HEL under the insulin promoter (IP-HEL) (37, 39). Results were identical in lightly irradiated and non irradiated mice. PTX refers to Pertussis toxin. STX refers to streptozotocin.

^‡

B10.BR refers to CD4 T cells from normal mice, incubated or not incubated with concanavalin A (ie activated and non-activated, respectively).

BDC2.5 refers to the diabetogenic CD4 T cells isolated from T cell receptor transgenic mice (36). Cells were activated by incubation with a mimotope peptide.

^‡

B6.g7 refers to CD4 T cells isolated from non-diabetic B6.g7 mice.