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. Author manuscript; available in PMC: 2013 Nov 21.
Published in final edited form as: J Am Chem Soc. 2012 Nov 6;134(46):19017–19025. doi: 10.1021/ja306035v

Figure 6.

Figure 6

Identification of in situ targets of probe 3. (A) A schematic representation of the in situ photo-crosslinking experiments performed with probe 3 in A431 cells. A table listing kinases that were specifically enriched is in the Supporting Information (Table S3, SI). (B) (left panel) EPHA2-V5-transfected and non-transfected COS-7 cells were photo-crosslinked with probe 3 (1 µM). Lysate obtained from both samples was conjugated to rhodamine-azide, resolved by SDS-PAGE, and labeled proteins were detected with in-gel fluorescence scanning. (right panel) Western blot analysis of EPHA2-V5-transfected and non-transfected COS-7 cells (anti-V5 antibody).