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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Calcif Tissue Int. 2012 Oct 9;91(6):379–386. doi: 10.1007/s00223-012-9655-9

Figure 5. Matrix mineralization of SM22α-Rankltg SMCs.

Figure 5

(A) Matrix calcium, normalized for total protein, in Rankltg and WT SMCs treated with control vehicle, beta-glycerophosphate (BGP, 5 mM), forskolin (Fsk, 25μM) or inorganic phosphate (Pi, 3 mM) for 7 days. (B) Matrix calcium, normalized for total protein, in Rankltg and WT SMCs treated with inorganic phosphate (Pi, 3 mM), and/or levamisole (Leva, 100 μM) for 10 days. (C) Matrix calcium, normalized for total protein, in Rankltg and WT SMCs treated with forskolin (Fsk, 25 μM) and/or levamisole (Lev, 100 μM) for 13 days. **p = 0.05; *p < 0.05; §p < 0.01; #p < 0.001.