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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Nat Chem. 2012 Oct;4(10):832–839. doi: 10.1038/nchem.1451

Figure 6. Tagging yeast cells with the GRG barcodes as in situ imaging probes.

Figure 6

(a) Cartoon illustrating the tagging mechanism. The biotinylated barcodes are anchored on the yeast cell through streptavidin molecules bound to biotinylated polyclonal antibodies coated on the yeast surface. Only two of the ten biotinylated staples on the barcode are shown for clarity. (b) Superimposed microscope images (acquired in bright field and TIRF) of the yeast cells treated with the barcodes. Top: yeast cells treated as illustrated in (a). Bottom: negative control: yeast cells treated with non-biotinylated barcodes. Scale bars: 5 μm.