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Activation properties of CFDA-Tz. (a) Activation of CFDA-Tz with various amounts of cell lysate. CFDA-Tz dye (20 µM) was incubated at room temperature with HT1080 cell lysate (0, 1, 2.5, 5, 10 or 20 µg). Fluorescence emission was recorded at 526 nm over time; (b) Emission spectrum of CFDA-Tz before and after activation by lysate. CFDA-Tz dye (20 µM) was incubated with HT1080 cell lysate (0 or 10 µg) for 40 min at room temperature. The fluorescence emission scan was then recorded.
