Figure 4.

A, Reelin as matrix induces morphological changes. Neurons were cultured on striped matrix of Reelin and control stripes for 36 h. Neurons were partially located on the Reelin stripe as visualized with the Reelin antibody G10 (green) and on the control stripes (scale bar, 10 μm). Axons growing on the Reelin stripe have longer axons and show more filopodia. B, Higher magnification of the F-actin staining of the Reelin stripe; between the two green lines, for better visualization of the axonal filopodia without the Reelin-staining (scale bar, 10 μm). C, Quantification of axonal length, total length of minor processes, axonal filopodia, minor processes, and minor process filopodia. Means ± SEM; n ≥ 27; **p < 0.01 vs controls. D, Reelin activates Cdc42 in organotypic rat hippocampal slices cultures. Slice cultures were treated with Reelin (30 min); afterward GTP-Cdc42 levels were determined by a PAK-CRIB pull-down assay. As input control, actin and Cdc42 were determined by Western blot. Contr., Control; proc., process.