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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: J Immunol. 2012 Feb 10;188(6):2669–2676. doi: 10.4049/jimmunol.1100529

Figure 2. Exogenous human (h)IL-2 cannot compensate for the IL-9-suppressive effect of SialoL.

Figure 2

(A) Naïve CD4+ T cells from BALB/c mice were stimulated (anti-CD3/CD28) in the presence or absence of different concentrations of sialoL (0.75 μM, 1.5 μM, 3 μM) under Th9-skewing conditions. Production of IL-2 was determined by ELISA. (B) Naïve CD4+ T cells from BALB/c mice were stimulated (anti-CD3/CD28) solely under Th9-skewing conditions or in the presence of neutralizing murine (m)IL-2 mAb (S4B6.1: 20μg/ml) or human(h)IL-2 (100ng/ml) and a combination of both. (C) Naïve CD4+ T cells from BALB/c mice were stimulated (anti-CD3/CD28) solely under Th9-skewing conditions or in the presence of sialoL (3 μM) or hIL-2 (100ng/ml) and a combination of both. IL-9 was determined after 72h by ELISA. Shown is one representative from two independent experiments.