Skip to main content
. Author manuscript; available in PMC: 2013 Feb 22.
Published in final edited form as: J Am Chem Soc. 2012 Feb 10;134(7):3346–3348. doi: 10.1021/ja2115663

Figure 2.

Figure 2

A microfluidic sample chamber containing two sensors supports the detailed measurement of the TF TBP binding in ~10 ul samples. a, the microfluidic chips used in this study. b, the dose-response behavior of the electrochemical TF sensor to the protein TBP is robust and sensitive in buffer or in 250 μg/ml HeLa nuclear extract (NE), displaying an affinity of 121 ± 20 nM. c, Endogenous TBP levels in nuclear extract can be quantitatively obtained using Eq. 1, comparing sensor response in extract to response in pure buffer (Smin, baseline signal) and in the presence of saturating levels of TBP (Smax, maximum response). NE analyzed by this method was shown to contain 4 ± 2 nM of TBP.