Fig. 3.
Cy5-ODN uptake by HDFs. (a-f) Cells were seeded on glass cover slips (control) with the Cy5-ODN/CPP complexation at molar ratios between 1/40 and 1/80. Scale bar = 100 µm. (g–l) Cells were seeded on Cy5-ODN/CPP scaffolds at volume ratios between 1/2 and 1/4. Scale bar = 50 µm. Pseudo color red indicates the distribution of Cy5-ODN, blue signals present cell nuclei by DAPI staining and green signals show cytoskeleton by Phalloidin staining. (m) Quantitative analysis of Cy5 signal intensity revealed significantly higher Cy5-ODN uptake on scaffolds than on cover slips. * indicates p < 0.05.