. Author manuscript; available in PMC: 2014 Jan 1.

Published in final edited form as: Acta Biomater. 2012 Oct 2;9(1):4513–4524. doi: 10.1016/j.actbio.2012.09.029

Table 1.

Processing parameters for siRNA-nanofiber scaffolds

Samples	Volume ratio^a	Equivalent molar ratio^b	Electrospinning parameters
Samples	Volume ratio^a	Equivalent molar ratio^b	Random fibers	Aligned fibers^d
siCOL1A1/MPG, siNEG/MPG	1/2	1/15	+12/-4 kV 1.5 mL/h 12 cm^c 5 × 5 cm²	-
	1/3	1/20		-
	1/4	1/30		2500 rpm^e
siCOL1A1/CADY, siNEG/CADY	1/2	1/15		-
	1/3	1/20		-
	1/4	1/30		2500 rpm^e
siCOL1A1/TKO, siNEG/TKO	1/2	1/15		2500 rpm^e
PCLEEP (plain fibers)	-	-		2500 rpm^e

Volume ratios of 1/2 (15µL/30µL), 1/3 (15µL/45µL) and 1/4 (15µL/60µL)

Conversion of volume ratios to molar ratios

Distance from needle tip to collector surface

All parameters were the same as random fibers except for the inclusion of a grounded rotating target

A grounded rotating target