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. 2012 Nov 19;109(49):19983–19987. doi: 10.1073/pnas.1214346109

Fig. 3.

Fig. 3.

Diphthine accumulates in Δylr143w yeast strain. (A) Labeling of eEF-2 purified from various strains using Rh-NAD and DT. The strains used are specified above each lane. In lanes 1–4, low DT concentration (0.1 μM) was used. In lanes 5–8, high DT concentration (10 μM) was used. (B) Diphtheria toxin sensitivity assay. The strains used are specified at the top. Plasmids used in the transformation are listed to the right. The cells were grown on 2% galactose. Each row represents a serial dilution from left to the right. (C) The mass spectrum of the eEF-2 peptide containing diphthine from the Δylr143w strain. (D) Ectopic expression of YLR143W or human ATPBD4 restores diphthamide biosynthesis in the Δylr143w strain. The plasmids used are shown above each lane. HsATPBD4 is the human ATPBD4. PhATPBD4 is the ortholog from P. horikoshii (PH1257). Low DT concentration (0.1 μM) was used in all lanes.