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Functional replacement of siRNA-depleted BubR1 with an AID-tagged BubR1 transgene. (A) Schematic outlines the experimental strategy used to replace endogenous BubR1 with GFP-AIDBubR1. (B) Immunoblot shows the levels of various proteins at 48 h after siRNA depletion of endogenous BubR1. (C) Graph shows the fraction of cells remaining in mitosis at various times after addition of nocodazole. There were >95 cells per condition from two independent experiments. Dox, doxycycline.
