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. 2012 Nov 19;109(49):20059–20064. doi: 10.1073/pnas.1213344109

Fig. 3.

Fig. 3.

Growth assays confirm bioinformatic predictions of siderophore biosynthesis and utilization capabilities. (A and B) Growth of producer strains 5F-79 and 12B01 in M9-based media (Fe+) and in media supplemented with 100 µM EDDA (Fe−), an iron-specific chelator. The results show that both siderophore producer strains 5F-79 (aerobactin) and 12B01 (vibrioferrin) are insensitive to EDDA addition. By contrast, nonproducers ZF-90 and ZF-264 (C and D), which have specific receptors for aerobactin (aer) and vibrioferrin (vf), respectively, suffered an appreciable effect when grown in Fe− media (100 µM EDDA for ZF-90, 50 uM EDDA for ZF-264). This effect was counteracted by addition of the corresponding siderophore aerobactin (80 µM) or vibrioferrin (70 µM) and by cell-free extract from corresponding producers 5F-79 and 12B01.