Skip to main content
. 2012 Nov 5;109(49):E3340–E3349. doi: 10.1073/pnas.1208618109

Fig. 2.

Fig. 2.

The ATP hydrolysis rate reflects the length-dependent RNA binding by MDA5. (A) ATP hydrolysis rates of MDA5 (0.3 μM) and RIG-I (30 nM) with model dsRNAs prepared by in vitro transcription (▲) and rotavirus genomic dsRNAs (●) (4.8 μg/mL) (mean ± SD, n = 3). (B) Relative ATP hydrolysis rate of MDA5 with rotavirus dsRNAs at different concentrations of RNA and MDA5 (mean ± SD, n = 4). Rates were normalized against the rate measured with 3.3 kb dsRNA. (C) Relative ATP hydrolysis rates of MDA5 (0.3 μM) with 112- or 1,012-bp dsRNA (4.8 μg/mL) containing variant 5′ functional groups, different sequences (Table S1), and different internal structures (a mismatch, bulge, or gaps) (mean ± SD, n = 3–4). Rates were normalized against the rate measured with intact 112-bp or 1,012-bp dsRNAs.