Figure 3.

Everolimus inhibited glycolysis in Panc-1 pancreatic cancer cells. Panc-1 human pancreatic cancer cells were treated with everolimus (10 μg/ml) for 6, 12 and 24 h. Panc-1 cells that were not treated with everolimus were used as a control. The LDH activity and lactate synthesis in each group were measured as described in Materials and methods. The levels of LDH activity and lactate synthesis decreased significantly following treatment with everolimus for 12 and 24 h. ^*P<0.05, compared with the control. LDH, lactate dehydrogenase.