Figure 4.

Everolimus suppressed the protein expression of mTOR. In the experimental groups, Panc-1 cells were treated with everolimus (10 μg/ml) for 6, 12 and 24 h. Panc-1 cells which were not treated with everolimus were used as a control. Each group of Panc-1 cells was lysed and western blotting was performed with p-S6 kinase and p-mTOR antibodies. Immunoblotting by anti-tubulin was performed for the control. mTOR, mammalian target of rapamycin.