Figure 5.

Everolimus upregulated miR-143 and downregulated HK2 in Panc-1 cells. After treatment with everolimus (10 μg/ml) for 6, 12 or 24 h, the relative expression of miR-143 and its target gene HK2 in Panc-1 cells were detected using real-time RT-PCR. The histogram plots demonstrate the relative expression levels of miR-143 and HK2 in each group. The levels of miR-143 and HK2 changed significantly following treatment with everolimus for 12 and 24 h. ^*P<0.05, ^**P<0.01, compared with the control. HK2, hexokinase 2; RT-PCR, reverse transcription-polymerase chain reaction.