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Transmission light microscope image of combined treatment in the COMB group of HSC-3 cells. (A), DAPI; (B), Annexin V; (C), propidium iodide (PI); (D) merged images. Fluorescein isothiocyanate (FITC)-Annexin V-positive and PI-negative cells were considered to be early apoptosis (arrows), while FITC-Annexin V-positive and PI-positive cells were considered to be secondary necrosis.
