Table 5. Susceptibilities of C. jejuni 11168 and its efflux mutants to the selected plant extracts in the presence or absence of PAβN (20 µg/mL)a, NMP (100 µg/mL) a, verapamil (100 µg/mL), reserpine (100 µg/mL) or CCCP (0.25µg/mL)b.
Extract ±inhibitor | 11168 | 11168B | 11168F | 11168R | ||||
MIC (µg/mL) | Fold diff. | MIC (µg/mL) | Fold diff. | MIC (µg/mL) | Fold diff. | MIC (µg/mL) | Fold diff. | |
I18 | 313 | 19.5 | 625 | 156 | ||||
+PAβN | <0.6 | >512 | 19.5 | 1 | 39 | 16 | 39 | 4 |
+NMP | 19.5 | 16 | 39 | 0.5 | 313 | 2 | 19.5 | 8 |
+Verapamil | 39 | 8 | 19.5 | 1 | 625 | 1 | 156 | 1 |
+Reserpine | 19.5 | 16 | 4.9 | 4 | 625 | 1 | 156 | 1 |
+CCCP | 4.9 | 64 | 9.8 | 2 | 313 | 2 | 156 | 1 |
V40 | 78 | 9.8 | 156 | 156 | ||||
+PAβN | 4.9 | 16 | <0.3 | ≥32 | 9.8 | 16 | 9.8 | 16 |
+NMP | 19.5 | 4 | <4.9 | ≥2 | 78 | 2 | 78 | 2 |
+Verapamil | 39 | 2 | 04.9 | 2 | 156 | 1 | 156 | 1 |
+Reserpine | 39 | 2 | 9.8 | 1 | 78 | 2 | 78 | 2 |
+CCCP | 4.9 | 16 | 9.8 | 1 | 78 | 2 | 156 | 1 |
V70 | 78 | 4.5 | 78 | 78 | ||||
+PAβN | 19.5 | 4 | 9.8 | 0.5 | <1.2 | ≥64 | 78 | 1 |
+NMP | 39 | 2 | 9.8 | 0.5 | 19.5 | 2 | 39 | 2 |
+Verapamil | 78 | 1 | 9.8 | 0.5 | 78 | 1 | 156 | 0.5 |
+Reserpine | 78 | 1 | 9.8 | 0.5 | 78 | 1 | 156 | 0.5 |
+CCCP | 39 | 2 | 4.5 | 1 | 39 | 2 | 78 | 1 |
A40 | 156 | 2.4 | 156 | 313 | ||||
+PAβN | 39 | 4 | 0.3 | 8 | 78 | 2 | 39 | 8 |
+NMP | 78 | 2 | 0.3 | 8 | 39 | 4 | 39 | 8 |
+Verapamil | 156 | 1 | 1.2 | 2 | 313 | 0.5 | 313 | 1 |
+Reserpine | 156 | 1 | 2.4 | 1 | 313 | 0.5 | 313 | 1 |
+CCCP | 39 | 4 | 2.4 | 1 | 156 | 1 | 313 | 1 |
Babić | 8,000 | 4,000 | 8,000 | 8,000 | ||||
+PAβN | 2,000 | 4 | 250 | 16 | 500 | 8 | 500 | 8 |
+NMP | 1,000 | 8 | 1,000 | 4 | 2,000 | 2 | 2,000 | 2 |
+Verapamil | 8,000 | 1 | 2,000 | 2 | 4,000 | 1 | 2,000 | 2 |
+Reserpine | 8,000 | 1 | 4,000 | 1 | 4,000 | 1 | 2,000 | 2 |
+CCCP | 125 | 64 | 125 | 32 | 125 | 32 | 125 | 32 |
“Fold diff.” indicates fold difference, which is calculated using the formula: MIC without an EPI/MIC with an EPI. ≥4-fold changes are indicated in bold.
PAβN and NMP significantly (p<0.05) reduced the MICs of the plant extracts in 11168, 11168F and 11168R, but not in 11168B (p > 0.05).
The effect of CCCP on the MICs of the plant extracts was only significant with 11168 (p<0.05).