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. 2012 Oct 30;5(1):77–84. doi: 10.3892/etm.2012.781

Figure 2.

Figure 2

Exogenous miR-145 influences the proliferation and invasion capacity of the human choriocarcinoma cells. (A) Methyl-thiazolyl-tetrazolium (MTT) assays showing the survival rate in various cells. The survival rates of miR-145-transfected (Aa) JAR cells and (Ab) JEG-3 cells were markedly lower than those of the corresponding untransfected and mutant miR-145 transfected cells, at 3 and 5 days post-transfection. (*P<0.05 vs. untransfected cells; **P<0.01 vs. untransfected cells; #P>0.05 vs. untransfected cells; n=3). (B) The results of the transwell migration invasion assay. The numbers of invading cells were significantly lower for miR-145-transfected (Ba) JAR cells and (Bb) JEG-3 cells than for the corresponding untransfected and mutant miR-145-transfected cells. (*P<0.05 vs. untransfected cells; #P>0.05 vs. untransfected cells; n=3). (C) The results of soft agar colony formation assay. Soft agar colony formation assays consistently indicated that miR-145-transfected (Ca) JAR cells and (Cb) JEG-3 cells formed substantially fewer colonies than the corresponding control or mutant miR-145-transfected-cells, when plated at low density. (*P<0.05 vs. untransfected cells; #P>0.05 vs. untransfected cells; n=3). These results suggest that miR-145-mediated repression of Sox2 expression significantly attenuates the invasion and migration capacity of human choriocarcinoma cells.