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. 2012 Oct 26;5(1):51–56. doi: 10.3892/etm.2012.771

Figure 3.

Figure 3

Induction of apoptosis of SGC-7901 cells following treatment with FAFs. After incubation with various concentrations of FAFs for 48 h, the cells were stained with Hoechst 33258 and PI dye and observed using fluorescent microscopy. 1, Control cells; 2, cells incubated with FAFs (4 μg/ml); 3, FAFs (8 μg/ml); 4, FAFs (16 μg/ml) and 5, FAFs (32 μg/ml). Apoptotic cells were detected and visualized by fluorescence microscopy using Hoechst 33342 and PI staining. Healthy cells (stained with Hoechst 33342) emitted blue fluorescence, and apoptotic cells (stained with Hoechst 33342 and PI) emitted red fluorescence (arrows). Scale bar, 100 μm. FAFs, Flos Albiziae flavonoids; PI, propidium iodide..