Figure 4. Indirect calorimetry, plasma ketones, and hepatic glycogen.

O₂ consumption (A, B), and CO₂ production (C, D) were measured by indirect calorimetry in chow- or HFD-fed animals over consecutive dark and light cycles. Respiratory exchange ratio (RER) was calculated as VCO₂/VO₂ (E, F). Fasting plasma ketones were measured after an overnight fast (G). Hepatic glycogen (H) was measured in chow-fed and HFD-fed Ogg1^−/− mice. n = 6 in each group; *, p<0.05 vs. WT.