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. 2012 Dec 17;7(12):e52280. doi: 10.1371/journal.pone.0052280

Figure 1. Construction of the miRNA-mowers.

Figure 1

We constructed miRNA-mowers by inserting multiple miRNA binding sites into the 3′ UTR of a Renilla luciferase gene. Each construct under control of a SV-40 promoter ended with a SV40 polyadenylation signal. An unregulated TK promoter-driven gene encoding firefly luciferase was used as a control. The black squares represented the linkers between the binding sites.