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. 2012 Dec 17;7(12):e51913. doi: 10.1371/journal.pone.0051913

Figure 2. UVI31+ overexpression physiology in E. coli and characterization of its endonuclease activity.

Figure 2

(A) Morphology of E. coli BL21 cells. Phase contrast micrographs of BL21 (pQE30); and BL21 over expressing UVI31+ (pRKM201). (B) UV survival of E. coli BL21 strains harboring the plasmid pRKM201 over expressing UVI31+ (▪)and pQE30 (•). (C) Endonuclease activity of UVI31+.Plasmid DNA un-irradiated (lane 1) or irradiated with 7.2 J/m2 of UV-C (lane 3) was incubated with 13 µM of UVI31+ respectively (lanes 2 and 4), for 30 min at 37°C. Plasmid DNA (lane 5) was incubated with 13 µM of UVI31+ (lanes 6–8) for 5, 15 and 30 min, respectively. M, 1 kb DNA ladder.