Figure 3. Expression of the P2X7 receptor in PSCs.

A. PCR results from PSCs isolated from WT and KO mice illustrate the presence of all isoforms A–D and K; mRNA transcripts are also present in KO. The area disrupted by homologous recombination (In) is, however, absent. Similarly, the area spanning the disrupted area (Sp) is missing. B. Immunostaining of PSCs with primary antibody for the extracellular domain of the P2X7 receptor. Scale bar is 10 µM. C. Western blots on whole cell lysate with antibody against an extracellular domain (ex-dom) and the C-terminal part of P2X7 (C-term) in reducing (r) and non-reducing conditions. The loading control was αSMA and this blot was exposed for shorter times. Representative of 3 independent experiments.