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. 2012 Dec 17;7(12):e51164. doi: 10.1371/journal.pone.0051164

Figure 4. Cell death of PSCs in the presence of 5 mM ATP.

Figure 4

A. Behavior of PSCs isolated from WT and KO was monitored for 10 hours; and pictures were taken at 2 min intervals. A. Representative phase contrast images of the PSCs at 0 hours, 5 hours and 10 hours. The top lane are WT cells and the bottom lane are KO cells. B. The bar graph summarizes the number of cells that died in control conditions or after addition of 5 mM ATP (n = 4–5 independent experiments). C. AnnexinV-FITC and propidium ioidide stain was added 5 hours after addition of 5 mM ATP to WT cells. D. Caspase 3/7 activation 5 hours after cells were stimulated with 5 mM ATP. E. BrdU incorporation measured 48 hours after the addition of 5 mM ATP. BrdU incorporations were normalized to 1% serum conditions. Significant difference (P<0.05) from respective controls (#) and between WT and KO (*) is indicated.