Figure 9. Calcium responses to BzATP and ATP in WT and KO PSC.

The Fluo-4 response (Ft/F0) of WT (A–D) and KO (E–H) PSCs stimulated with 50 µM BzATP, 50 µM ATP and lastly with 5 µM ionomycin. Intracellular Ca²⁺ signals were monitored in control conditions (A, E), in presence az101606120 (B, F), suramin (C, G) and suramin together with az10606120 (D, H). The figures illustrate one independent representative run with the frame average for about 50 cells in black, and an example response of 5 individual cells is shown in color. The peak intracellular Ca²⁺ responses to agonists +/− antagonists derived from frame intensities for approximately 50 cells/preparation were averaged for 4 separate PSCs preparations form WT and KO mice and shown as bargraphs. The letters underneath the bars show from which experiment (indicated by a letter) the mean Ca²⁺ peak values were taken from. Significant difference (P<0.05) from respective controls (#) and in between WT and KO preparations (*) is indicated.