Table 1. Primer sets used for RT-PCR on PSCs for isoforms A–D and K, and within the deleted area (Intra) and spanning the deleted area (Spanning).
Primers | Sequence | Product length |
P2X7 A FW | TGCACTCTTGAGGAGCGCCGA | 218 |
P2X7 A RW | TGATTCCTCCCTGAACTGCCACCT | |
P2X7 B FW | GCCGCACATTCGCATGGTGG | 247 |
P2X7 B RW | TGGCTGAATGGTTACGACCACTTGCT | |
P2X7 C FW | TGCTTTCTGCAGGTCGGGGGT | 228 |
P2X7 C RW | GAAACAAGTATCTAGGTTGGAACTTCTTGGCC | |
P2X7 D FW | ACACCTTCCCTTTGCAGGGGAACT | 197 |
P2X7 D RW | GGCAGCAGAACTTTAGGCCAGCT | |
P2X7 K FW | GGATCGGGACGCTGAAGAACAC | 173 |
P2X7 K RW | CTTCGTCACCCCACCCTCTGTGA | |
P2X7 Intra FW | TCCAAGCTCTTCCATAAGCTCGTGC | 70 |
P2X7 Intra FW | AGGGATCCTGGTAAAGCAGGAGGAG | |
P2X7 Spanning FW | GCCCGAGTTGGTGCCAGTGT | 223 |
P2X7 Spanning FW | TGTCGCAGCCTGCTGTTGGT |