Figure 2. Influenza-specific IgG and hemagluttination inhibition (HAI) titers elicited by unadjuvanted and GPI-0100-adjuvanted mucosal influenza vaccine.

Serum samples from the mice described in the legend to Fig. 1 were collected on day 20 and day 34. (A) Total IgG responses after a single immunization. Average 10log IgG titers ± standard error of the mean (S.E.M.), n  = 6 mice per group. The detection limit of the assay is represented by the dotted line. (B) Total IgG responses after two immunizations. (C) HAI titers after two immunizations. The results are expressed as 2log HAI titers of individual mice. The black line represents the geometric mean virus titer per group. Due to technical reasons only 5 and 4 samples from mice receiving 30 µg GPI-0100 adjuvanted intranasal immunization and unadjuvanted intrapulmonary immunization were available for the HAI assay.