Figure 4. Effect of GPI-0100 on influenza-specific IL-4-producing T cells.
Mice were immunized on day 0 and on day 20 with 1 µg A/PR/8 subunit vaccine alone or adjuvanted with the indicated doses of GPI-0100. The immunizations were given via intramuscular (i.m.), intranasal (i.n.) or intrapulmonary (pul.) route. Spleen samples were collected one week later upon termination. Splenocytes were isolated and stimulated overnight with PR8 subunit. The result is expressed as cytokine producing splenocytes per 5×105 cells of individual mice.