Table 1. Bacterial, worm strains and plasmids used in this study.
| Strain/Plasmid | Description | References |
|---|---|---|
|
B. pseudomallei | ||
| R15 |
Wild-type clinical isolate; GmR |
19 and 51 |
| R15-GFP |
Isogenic strain of R15 transformed with pSMompAgfp; GmR CmR |
This study |
|
E. coli | ||
| TOP10 |
F–mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(ara leu) 7697 galU galK rpsL (SmR) endA1 nupG |
Invitrogen |
| OP50 |
Derivative of E. coli B strain; uracil auxotroph; lacks O-antigen component of its outer membrane; SmR |
Caenorhabditis Genetics Center (CGC), 52 |
|
cdc-25.1 RNAi clone |
E. coli HT115(DE3) expressing dsRNA complementary to C. elegans cdc-25.1 gene; CbR |
48 |
|
pgp-5 RNAi clone |
E. coli HT115(DE3) expressing dsRNA complementary to C. elegans pgp-5 gene; CbR |
Open Biosystems |
|
C. elegans | ||
| Bristol N2 |
Wild-type nematode isolated from mushroom compost near Bristol, England. |
CGC |
|
tnt-3(aj3) |
WE5006; contains a recessive mutation in the troponin T gene expressed in the pharyngeal muscles |
32 and 33 |
| ppgp-5::gfp |
WE5172; contains an integrated transgene ppgp-5::gfp (transgene constructed by Baillie Laboratory, Simon Fraser University) |
38 |
|
rrf-3(pk1426);glp-4(bn2) |
WE5134; contains loss-of-function mutations in the rrf-3 and glp-4 genes; exhibits germ line proliferation defect at 25°C and enhanced RNAi sensitivity |
53 and 54 |
|
Plasmids | ||
| pSM202 |
Derivative of pBRR1MCS; 3.686 kb; bears rep, mob, cat (CmR) genes and two multiple cloning sites (MCS 1 and 2). |
Mohamad S, unpublished data |
| pSMompAgfp | pSM202 with the PompA::gfp construct inserted into the MCS2; ~4.62 kb | This study |
Abbreviations: Cb, carbenicillin; Cm, chloramphenicol; Gm, gentamicin; Sm, streptomycin.