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. 2012 Oct 1;3(6):529–533. doi: 10.4161/viru.21540

Table 4. PCR program setup.

 
 Program 1 (Mix 1, 2 and 3)
 Program 2 (Mix 4)
  Temperature Time Temperature Time
Initial denaturation
 95°C
 3 min
 95°C
 3 min
Denaturation
 95°C
 15 sec
 95°C
 15 sec
Annealing
 60°C
 20 sec
 52.5°C
 45 sec
Elongation
 72°C
 2 min
 72°C
 3 min
Final elongation 72°C 7 min 72°C 7 min