Figure 1. HER2-Akt signaling regulates Aurora B stability. (A) HER2 overexpression positively regulates the steady-state expression of CSN6. Indicated equal amounts of cell lysates were immunoblotted with indicated antibodies. (B) Akt positively regulates the steady-state expression of CSN6. 293T cells were co-transfected with indicated plasmids and increasing amounts of Akt. Equal amounts of cell lysates were immunoblotted with indicated antibodies. (C) Akt-overexpressing cells have increased steady-state expression of CSN6. Indicated amounts of cell lysates were immunoblotted with indicated antibodies. (D) Antagonizing Akt leads to destabilization of CSN6 in HER2-overexpressing cells. Indicated amounts of cell lysates were immunoblotted with indicated antibodies. (E) Dmominant Akt reduces the expression levels of CSN6. Indicated amounts of cell lysates were immunoblotted with indicated antibodies. (F) Inhibitor of PI3K-Akt pathway causes destabilization of CSN6. Rat1-Akt cells were treated with LY294002 for 6 h before harvesting. Equal amounts of cell lysates were immunoblotted with indicated antibodies.