Figure 2. Akt induces CSN6 nuclear import. (A) CSN6 nuclear localization is induced in the presence of Akt. U2OS cells were transfected with indicated plasmids. Nucleus was stained with Hoechst 33342 and pseudo colored with red. (B) CSN6 is localized at the nucleus at Akt-overexpressing cells. Indicated cells were stained with Anti-Csn6 followed with FITC-conjugated secondary Ab. Nucleus was stained with DAPI.