Figure 6A-C. CSN6 S60D mutant efficiently induces p53 degradation and enhances transformation. (A) CSN6 reduces the levels of MDM2 ubiquitination. 293T cells were transfected with the indicated plasmids. The proteasome inhibitor MG132 was added 6 h before harvesting cells. The ubiquitinated amount of MDM2 is analyzed by immunoprecipitated (IP) with anti-MDM2 followed by immunoblotting (IB) with anti-ubi. (B) CSN6 S60D mutant efficiently degrades p53. A549 cells were transfected with indicated plasmids and equal amounts of cell lysates were were immunoblotted with indicated antibodies. (C) Akt enhances CSN6-mediated cell transformation. Cells were transfected with indicated plasmids, and foci-formation assay was performed.