Figure 3. Ada3 maintains genome integrity. (A) Representative metaphase images for spontaneous chromosome abnormalities in Ada3fl/fl and Ada3−/− MEFs (arrow indicates one of the aberration seen upon Ada3 deletion). (B) Western blotting for Ada3 protein in Ada3fl/fl and Ada3−/− primary MEFs shows the adeno-cre deletion of Ada3; β-actin was used as a loading control. (C) Summary for spontaneous genomic instability in Ada3fl/fl and Ada3−/− primary MEFs. Metaphase spreads were Giemsa banded and analyzed for chromosomal abnormalities.