Figure 5. Ada3 deletion followed by IR induces more chromosomal aberrations in S phase of the cell cycle. (A) For G₁-specific aberrations, cells in plateau phase of proliferation were irradiated with 3 Gy, incubated for 24 h post-irradiation and then subcultured, and metaphases were examined. (B) For S-phase-specific aberrations cells in exponential phase were irradiated with 2 Gy and analyzed after 4 h post IR, as above, (C) For G₂ phase-type aberrations, cells in exponential phase were irradiated with 1 Gy and analyzed as above. For each experiment, 50 metaphases were scored. Data represents mean ± SE of three independent experiments, each done in triplicates.