Figure 6. Ada3 deletion delays DNA repair process. Ada3fl/fl and Ada3−/− immortalized MEFs were irradiated with 10 Gy of γ irradiation and analyzed for DNA damage-induced comet tail over various time periods (1–16 h) after irradiation. (A) Representative images of neutral comet assays in both cells. (B) Unrepaired DNA was quantified as comet tail length (in pixels) using the CometScore software. At least 50 cells per sample were analyzed, and presented as mean ± SE from three independent experiments.