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. 2012 Dec 17;7(12):e51482. doi: 10.1371/journal.pone.0051482

Table 2. Species-specific quantitative PCR (qPCR) primers.

Species Gene Primer Direction Sequence (5′-3′) Product Size (bp)
Golden-collared Manakin PV Forward GAAGGGCTTTACCCCAGAAG 72
Reverse TTATCTCCAGCAGCCAGAAG
IGF-I Forward AGGAGGCTGGAGATGTACTGTG 103
Reverse GCACTTCCTTTTGTGCTTTTG
MyoD Forward GGAATCACCAAATGATCCAAAG 74
Reverse CTACAATGCTTGAGAGGCAATC
MSTN Forward TGAACCCATTTTTAGAGGTCAG 85
Reverse GTGGAGTGCTCGTCACAGTC
Zebra Finch PV Forward TTGTCCTGAAGGGCTTTACC 93
Reverse TACCATCACCGTCCTTATCTCC
IGF-I Forward AACCAGTTCTGTTGCTGCTG 89
Reverse AAAGCCTCTGTCTCCACACAC
MyoD Forward ACAGAGTCCCCAAATGATCC 72
Reverse AATGCTTGAGAGGCAATCG
MSTN Forward ACTGCGTCTGGAACAAGCTC 195
Reverse AGAAAATCAGACTCTGTAGGCATTG
Ochre-bellied Flycatcher PV Forward AAGTTTGTCCTGAAGGGCTTTAC 100
Reverse TTTTACCATCACCGTCCTTATC
IGF-I Forward GGCTGGAGATGTATTGTGCTC 98
Reverse CACTTCCTTTTGTGCTTTTGG
MyoD Forward AGCAGGAGGACGCTTATTACC 110
Reverse AGGAGGCCCGCTGTATTC
MSTN Forward GCGATGGCTCTTTGGAAG 75
Reverse AATCAGACTCCGTAGGCATTG