Table 2. Species-specific quantitative PCR (qPCR) primers.
Species | Gene | Primer Direction | Sequence (5′-3′) | Product Size (bp) |
Golden-collared Manakin | PV | Forward | GAAGGGCTTTACCCCAGAAG | 72 |
Reverse | TTATCTCCAGCAGCCAGAAG | |||
IGF-I | Forward | AGGAGGCTGGAGATGTACTGTG | 103 | |
Reverse | GCACTTCCTTTTGTGCTTTTG | |||
MyoD | Forward | GGAATCACCAAATGATCCAAAG | 74 | |
Reverse | CTACAATGCTTGAGAGGCAATC | |||
MSTN | Forward | TGAACCCATTTTTAGAGGTCAG | 85 | |
Reverse | GTGGAGTGCTCGTCACAGTC | |||
Zebra Finch | PV | Forward | TTGTCCTGAAGGGCTTTACC | 93 |
Reverse | TACCATCACCGTCCTTATCTCC | |||
IGF-I | Forward | AACCAGTTCTGTTGCTGCTG | 89 | |
Reverse | AAAGCCTCTGTCTCCACACAC | |||
MyoD | Forward | ACAGAGTCCCCAAATGATCC | 72 | |
Reverse | AATGCTTGAGAGGCAATCG | |||
MSTN | Forward | ACTGCGTCTGGAACAAGCTC | 195 | |
Reverse | AGAAAATCAGACTCTGTAGGCATTG | |||
Ochre-bellied Flycatcher | PV | Forward | AAGTTTGTCCTGAAGGGCTTTAC | 100 |
Reverse | TTTTACCATCACCGTCCTTATC | |||
IGF-I | Forward | GGCTGGAGATGTATTGTGCTC | 98 | |
Reverse | CACTTCCTTTTGTGCTTTTGG | |||
MyoD | Forward | AGCAGGAGGACGCTTATTACC | 110 | |
Reverse | AGGAGGCCCGCTGTATTC | |||
MSTN | Forward | GCGATGGCTCTTTGGAAG | 75 | |
Reverse | AATCAGACTCCGTAGGCATTG |