Fig. 2.

Representative plots showing leukocyte populations present after ICH. a Inflammatory macrophages (I.Mϕs) and Gr1⁻ macrophages (Gr1⁻ Mϕs) are both present in brain, but the inflammatory macrophages are much more numerous. This plot is gated on CD45.1^hiCD3⁻Ly6G⁻CD11c⁻ cells. b Plot showing CD11c^int (blue gate, center) and CD11c^hi dendritic cell populations (DCs, black gate, right) is gated on CD45.1^hiCD3⁻Ly6G⁻ cells. The majority of CD11c^int cells express Gr1 (inset) and are termed inflammatory dendritic cells. c Few neutrophils (PMNs) are found in brain at 12 hours. The plot is gated on CD45.1^hiCD3⁻. d T cells are found in the ipsilateral hemisphere by 12 hours. The plot is gated on CD45.1^hi cells. e Pie chart showing the distribution of leukocyte populations expressed as the mean percentage of infiltrating cells from 5 mice. “Other” indicates CD45.1^hi cells that do not fall into any of the aforementioned gating strategies