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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: J Food Prot. 2012 Apr;75(4):660–670. doi: 10.4315/0362-028X.JFP-11-480

Fig. 4.

Fig. 4

Sensitivity of the real-time PCR assay to detect six major foodborne pathogens: Aliquots of DNA from E. coli O157:H7 (a), S. dysentriae (b), F. tularensis ssp. tularensis (c), S. enterica ssp enterica s. Typhi (d), V. cholerae (e), and Y. pestis (f), were serially five-fold diluted and analyzed by real-time PCR to determine the lowest detection limit of the assay. At the end of the 27 cycles of PCR, aliquots of the reactions were also resolved by agarose gel electrophoresis to examine the PCR products and intensity from each dilution (lower inset panels).