Figure 1.
Pretreatment of mice with PTEN siRNA (2mg/kg i.v. at −4h) ameliorates liver IRI, compared with untreated or scramble siRNA-treated WT controls (6h of reperfusion after 90min warm ischemia).
(A) The sALT (IU/L) levels. *p<0.0005, n=4–6/group. Mean±SD are shown.
(B) Representative liver histology of ischemic liver lobes: (a) Sham; (b) untreated WT; (c) PTEN siRNA-treatment (d) scramble siRNA-treatment. (H&E staining; magnification ×100, n=4–6/group)
(C) Suzuki’s histological grading of liver IR-damage *p<0.0001, n=4–6/group. Mean±SD are shown.
(D) Liver neutrophil accumulation, assessed by MPO activity (U/gm). *p<0.01, **p<0.005. n=4–6/group. Mean±SD are shown.