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. Author manuscript; available in PMC: 2013 Dec 17.
Published in final edited form as: Chem Res Toxicol. 2012 Oct 16;25(12):2642–2653. doi: 10.1021/tx3002753

Table 1.

Summary of the nitrotyrosine-containing peptides identified in PN-treated CYP3A4a.

Position of
nitrated Tyr		 Modified peptide sequences m/z of
full mass		 Precursor
ion charge
99 97ECYSVFTNR105 1163.4 1
307 295LVAQSIIFIFAGYE308 1616.6 1
347 343APPTYDTVLQME354 1410.5 1
430 and 432 425DNIDYIYTPFGSSPR440 1903.0 2
a

PN-treated samples were digested by trypsin and/or Glu C and analyzed by LC-MS/MS as described in Materials and Methods. A mass shift of 45 Da due to nitration of each tyrosine in the peptides analyzed by LC-MS/MS was used for the SEQUEST database search (Lin et al., 2010).