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. Author manuscript; available in PMC: 2013 Dec 1.
Published in final edited form as: Mol Microbiol. 2012 Oct 24;86(6):1376–1392. doi: 10.1111/mmi.12063

Fig. 8. Haem-induced oxidative damage localizes primarily to the cell membrane.

Fig. 8

A. Cell membranes and cytoplasmic fractions were isolated from S. aureus strains grown in the presence or absence of 5 μM haem and analyzed for the presence of protein carbonylation by immunoblotting. The symbols “-” and “+” denote the absence or presence of 5 μM exogenous haem in the growth media. B. Quantification of immunoblots performed in triplicate representing the percent oxidative protein damage in haem-treated samples as compared to untreated samples. Error bars represent SEM. * denotes p < 0.05 as determined by a two-tailed Student’s t test.