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. Author manuscript; available in PMC: 2014 Feb 1.
Published in final edited form as: J Bone Miner Res. 2013 Feb;28(2):283–290. doi: 10.1002/jbmr.1747

Fig. 2.

Fig. 2

FasL is cleaved after E2 treatment. U2OS-ERα cells were transfected with Flag-vector alone (V), wildtype (WT) Flag-FasL or mutated Flag-FasL (ELAELR or SLELR) then treated with vehicle control (C) or 10 nM E2 for 24 hours. Cells were lysed and immunoblots were performed with anti-Flag and anti-actin antibodies.