Table 1. Entry of H. pylori into the deep mucus layer (juxtamucosal mucus) under three simulated postprandial conditions.
| Initial luminal bacterial number | Initial luminal bacterial number above 10,000 µm2 tissue (50 nl volume) | Number of H. pylori in juxtamucosal mucus above 10,000 µm2 tissue | Colonization ratio | ||
|---|---|---|---|---|---|
| motile H. pylori / nl | motile H. pylori / 10,000 µm2 | juxtamuscosal H. pylori / 10,000 µm2 | luminal H. pylori : juxtamucosal H. pylori | ||
| Gastric conditions | Animals | mean ± SD from all samples | mean ± SD all samples from each animal accumulated | ||
| profile B (“baby”) | n = 8 | 466 ± 198 | 23,281 ± 11,038 | 16 ± 8 | 2,500∶1 ± 3,200∶1 |
| profile C (“child / toddler”) | n = 10 | 393 ± 136 | 19,655 ± 6,822 | 128 ± 54 | 170∶1 ± 80∶1 |
| profile A (adult) | n = 5 | 475 ± 71 | 23,730 ± 3,559 | 27 ± 20 | 1,600∶1 ± 1,200∶1 |
| Significance | No statistically significant difference between all samples | C vs.B P = 0.0001 C vs.A P = 0.016 B vs.A n.s. | C vs.B P = 0.034 C vs.A P = 0.0036 B vs.A n.s. | ||
Note. The bacterial counts are expressed as mean ± SD across all samples. Mean ± SD of colonization rate was calculated from all samples of each experiment/ animal accumulated, in order to compare animals. Therefore the ratio of the average of displayed bacterial counts can differ from the average of all colonization rates for mathematical reasons.