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Effects of recombinant adenoassociated virus (rAAV)-mediated gene transfer on wake maintenance during the night. Individual wake bouts were grouped into bins of different durations. Data represent group means ± standard error of the mean (SEM). Orexin (ORX) knockout mice given rAAV-orexin into the dorsolateral pons had a significant increase in the percent of time spent in long bouts. *P < 0.05 versus no rAAV. GFP, green fluorescent protein; WT, wild-type.
